STAT-1 is activated upon IFNa/b, IFNg, or IL-6 binding to it"s cognate receptor. Once Stat1 is phosphorylated by JAKs it forms a homodimer, or heterodimer with STAT-2 and translocated to the nucleaus. Once in the nucleus the dimer binds to the GAS sequence (IFNg activation site) in the promoter region of target genes and induces transcription. STAT-1 can also be phosphrylated by numerous serine/threonine kinases, which increase transcriptional activity.
Cells will undergo genotypic changes resulting in reduced responsiveness over time in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages.
STAT1; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1-alpha/beta; ISGF 3; STAT91; transcription factor ISGF 3 components p91/p84; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; CANDF7; ISGF-3; DKFZp686B04100;
RNA polymerase II core promoter sequence-specific DNA binding; RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; calcium ion binding; double-stranded DNA binding; enzyme binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein homodimerization activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; NOT sequence-specific DNA binding transcription factor activity; signal transducer activity; tumor necrosis factor receptor binding;