Human STAT1 Knockout Cell Line-HEK293

Name: Human STAT1 Knockout Cell Line-HEK293
SKU: CSC-RT2745
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2745

Target Gene : STAT1 Host Cell : HEK293

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2745
Description	This cell is a stable cell line with a homozygous knockout of human STAT1 using CRISPR/Cas9.
Target Gene	STAT1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	STAT1 signal transducer and activator of transcription 1, 91kDa [ Homo sapiens ]
Gene Symbol	STAT1
Synonyms	STAT1; signal transducer and activator of transcription 1, 91kDa; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1-alpha/beta; ISGF 3; STAT91; transcription factor ISGF 3 components p91/p84; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; CANDF7; ISGF-3; DKFZp686B04100;
Gene ID	6772
Uni Prot ID	P42224
m RNA Refseq	BC002704
Chromosome Location	2q32.2-q32.3
Function	RNA polymerase II core promoter sequence-specific DNA bi
Pathway	Adipogenesis, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;
MIM	600555

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The STAT1 gene encodes the signal transducer and activator of transcription 1 protein, a key member of the STAT protein family. This gene plays a crucial role in mediating various cellular responses to cytokines and growth factors. When these ligands bind to their corresponding receptors, the receptor-associated kinase phosphorylates the STAT1 protein, which subsequently forms homo- or hetero-dimers. These dimers translocate to the nucleus and act as transcriptional activators to regulate gene expression. STAT1 is activated by a variety of ligands, including interferon-α, interferon-γ, EGF, PDGF, and IL6. This activation is essential for cell viability, immune responses to viral, fungal, and mycobacterial pathogens, and a variety of other cellular processes.

STAT1 mutations are associated with several immunodeficiency diseases, such as immunodeficiency 31A, 31B, and 31C. These mutations can result in either loss- or gain-of-function, each resulting in different clinical manifestations. Loss-of-function mutations hamper the immune system's ability to respond to infection, leading to increased susceptibility to viral and bacterial pathogens. In contrast, gain-of-function mutations are often associated with chronic mucocutaneous candidiasis (CMC), leading to an overstimulated immune response, resulting in recurrent infections, often involving Candida species, and are frequently associated with other autoimmune diseases.

The human STAT1 knockout cell line - HEK293 provides a valuable model for biological and medical research. The following are the main applications of this cell line:

Signal Transduction Studies: STAT1 protein plays a key role in the cellular response to cytokines and growth factors through the JAK-STAT pathway. Using STAT1 knockout HEK293 cells, researchers can study signaling mechanisms and pathway alterations in the absence of STAT1, gaining insight into the role of STAT1 in cellular processes.

Cancer Research and Drug Development: Given STAT1's involvement in tumor suppression and immune response regulation, STAT1 knockout cells are essential for understanding cancer cell behavior. These cells help determine the effects of STAT1 loss on tumorigenesis and test new anticancer drugs that target STAT-related pathways.

Infectious Disease and Immunology Research: STAT1 is essential in mediating the cellular response to viral and bacterial infections. Researchers use STAT1 knockout HEK293 cells to study how STAT1 loss affects immune responses, pathogen replication, and the effectiveness of antiviral treatments.

Gene Expression and Regulation: By examining changes in gene expression in STAT1 knockout cells, scientists can identify STAT1-dependent genes and regulatory networks. This helps map the transcriptional landscape affected by STAT1 and understand its role in gene regulation under various physiological and pathological conditions.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Helpful

By comparing the behavior of STAT1 knockout cells to normal cells, we can gain valuable insights into the role of STAT1 in cellular signaling. It is very helpful for our research.

United States

2021-05-12

Good experimental results

By studying STAT1 knockout cells, we can observe how the absence of STAT1 affects cancer cell growth, invasion, and response to therapy. I recommend Creative Biogene'cell line.

United States

2021-08-15

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Human STAT1 Knockout Cell Line-HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Helpful

Good experimental results

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