Human PRNP HEK293 Cell Lysate

Name: Human PRNP HEK293 Cell Lysate
SKU: CLOE-0688
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0688 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0688
Description	This cell lysate over-expresses human PRNP (NP_000302.1) (Met1-Gly229) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified.
Introduction	This cell lysate over-expresses human PRNP (NP_000302.1) (Met1-Gly229) fused with the Fc region of human IgG1. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	PRNP
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	PRNP prion protein [ Homo sapiens ]
Gene Symbol	PRNP
Synonyms	CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Gene Description	prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)
GeneID	5621
Uni ProtID	P04156
mRNA Refseq	NM_000311.3
Protein Refseq	NP_000302.1
Chromosome Location	20p13
Function	ATP-dependent protein binding; chaperone binding; copper ion binding; copper ion binding; identical protein binding; microtubule binding; protein binding; tubulin binding;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Glypican 1 network, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Prion diseases, organism-specific biosystem; Prion diseases, conserved biosystem;
MIM	176640

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