Human PRNP Knockout Cell Line-A549

Name: Human PRNP Knockout Cell Line-A549
SKU: CSC-RT2826
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2826

Target Gene : PRNP Host Cell : A549

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2826
Description	This cell is a stable cell line with a homozygous knockout of human PRNP using CRISPR/Cas9.
Target Gene	PRNP
Host Cell	A549
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid Nitrogen
Shipping	Dry ice package

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PRNP prion protein [ Homo sapiens ]
Gene Symbol	PRNP
Synonyms	CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Gene Description	prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)
GeneID	5621
Uni ProtID	P04156
mRNA Refseq	NM_000311.3
Protein Refseq	NP_000302.1
Chromosome Location	20p13
Function	ATP-dependent protein binding; chaperone binding; copper ion binding; copper ion binding; identical protein binding; microtubule binding; protein binding; tubulin binding;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Glypican 1 network, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Prion diseases, organism-specific biosystem; Prion diseases, conserved biosystem;
MIM	176640

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