Human PPARG lentiviral particles

Name: Human PPARG lentiviral particles
SKU: LV00431Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00431Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV00431Z
Description	Human PPARG ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type	Lentiviral particle
Gene	PPARG
Species	Human
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	PPARG peroxisome proliferator-activated receptor gamma [ Homo sapiens ]
Gene Symbol	PPARG
Synonyms	GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Gene Description	peroxisome proliferator-activated receptor gamma
GeneID	5468
Uni ProtID	P37231
mRNA Refseq	NM_015869.4
Protein Refseq	NP_056953.2
Chromosome Location	3p25
Function	DNA binding; RNA polymerase II regulatory region DNA binding; activating transcription factor binding; arachidonic acid binding; chromatin binding; drug binding; enzyme binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; prostaglandin receptor activity; protein binding; retinoid X receptor binding; contributes_to sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; transcription regulatory region DNA binding; transcription regulatory region DNA binding; zinc ion binding;
Pathway	Adipogenesis, organism-specific biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Energy Metabolism, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem;
MIM	601487

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