Human NFKB2 Knockout Cell Line-HeLa

Name: Human NFKB2 Knockout Cell Line-HeLa
SKU: CSC-RT0675
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0675

Target Gene : NFKB2 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0675
Description	A stable cell line with a homozygous knockout of human NFKB2 using CRISPR/Cas9.
Target Gene	NFKB2
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nitrogen
Shipping	10^6 cells/tube

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NFKB2
Gene ID	4791

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Stably

My design requires a large number of gene transfection experiments, using this product, my experiments went very well and saved a lot of time

United States

2020-02-08

Efficient

This NFKB2 Knockout Cell is better than the transfection reagents I used before, and the gene knockout efficiency is very efficient, greatly improving the success rate of the experiment.

United States

2021-12-02

Improved

This NFKB2 Knockout Cell does not have very big loopholes like previous products, off-target effects rarely appear, will not do the same experiment over and over.

United States

2022-02-06

Wide coverage

This NFKB2 Knockout Cell Line is easy to use, simple to construct and can cover most regions of the NFKB2 gene.

United States

2022-09-10

Highly efficient

This NFKB2 Knockout Cell Line is easy to handle. Highly efficient on the genome and does not require modification of the Cas9 nuclease.

United States

2023-03-01

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Human NFKB2 Knockout Cell Line-HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Stably

Efficient

Improved

Wide coverage

Highly efficient

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