Human NCSTN HEK293 Cell Lysate

Name: Human NCSTN HEK293 Cell Lysate
SKU: CLOE-0975
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-0975 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-0975
Introduction	This cell lysate over-expresses extracellular domain of human NCSTN (NP_056146.1) (Met 1-Glu 669) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	NCSTN
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	NCSTN nicastrin [ Homo sapiens ]
Gene Symbol	NCSTN
Synonyms	APH2; RP11-517F10.1
Gene Description	nicastrin
Gene ID	23385
Uni Prot ID	Q92542
m RNA Refseq	NM_015331.2
Protein Refseq	NP_056146.1
Chromosome Location	1q22-q23
Function	endopeptidase activity; protein binding;
Pathway	Activated NOTCH1 Transmits Signal to the Nucleus, organism-specific biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Alzheimers Disease, organism-specific biosystem; Cell death signalling via NRAGE, NRIF and NADE, organism-specific biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; NRIF signals cell death from the nucleus, organism-specific biosystem;
MIM	605254

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