Human LRP6 Knockout Cell Line-HeLa

Name: Human LRP6 Knockout Cell Line-HeLa
SKU: CSC-RT0763
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT0763

Target Gene : LRP6 Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT0763
Description	A stable cell line with a homozygous knockout of human LRP6 using CRISPR/Cas9.
Target Gene	LRP6
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Storage	Liquid nitrogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LRP6
GeneID	4040

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

Low-density lipoprotein receptor-related protein 6 (LRP6) is a key protein involved in multiple cellular processes, including lipid metabolism, signal transduction, and cell development. Encoded by the LRP6 gene located on chromosome 12p13.2, the protein plays an important role in the canonical Wnt signaling pathway, which is essential for embryonic development, cell proliferation, and differentiation. In Wnt signaling, LRP6 forms a complex with the Frizzled receptor upon Wnt ligand binding. This interaction triggers the phosphorylation of LRP6, creating docking sites for other signaling molecules such as Disheveled (Dvl) and Axin. The subsequent stabilization of β-catenin and its translocation into the nucleus leads to the transcription of Wnt target genes, thereby affecting cell fate, proliferation, and migration. LRP6-mediated dysregulation of Wnt signaling has been implicated in various pathologies, including cancer, osteoporosis, and neurodegenerative diseases.

In addition to its role in Wnt signaling, LRP6 is also involved in lipid metabolism by regulating the clearance of plasma lipoproteins. Studies have shown that mutations in the LRP6 gene can lead to severe metabolic disorders such as familial hypercholesterolemia and coronary artery disease, highlighting its importance in maintaining metabolic homeostasis. Its importance in development and pathology makes it an important topic for biomedical research. Understanding the mechanistic basis of LRP6 function could pave the way for targeted therapeutic interventions in related diseases.

Because the LRP6 gene plays an important role in a variety of cellular and physiological processes, creating a human LRP6 knockout cell line in HeLa cells can be used for a variety of research applications. Here are some important applications:

Cancer Research: By observing the effects of LRP6 loss, researchers can gain insight into how LRP6 promotes tumor progression, metastasis, and cell proliferation, allowing for the development of targeted cancer therapies.

Wnt Signaling Pathway Analysis: LRP6 is an essential coreceptor in the Wnt signaling pathway. Knockout cell lines allow scientists to study the specific functions of LRP6 in this pathway and understand its role in cellular processes such as differentiation, migration, and growth.

Drug Testing and Development: Using LRP6 knockout HeLa cells in high-throughput screening assays can identify potential therapeutic compounds. These cells provide a platform to test the efficacy and safety of drugs that may modulate the Wnt signaling pathway or other pathways affected by LRP6 loss.

Genetic and Epigenetic Studies: This cell line can be used to explore genetic and epigenetic changes that occur due to LRP6 loss. Researchers can study gene expression profiles, chromatin modifications, and other alterations to gain deeper insights into gene regulation mechanisms.

Elucidation of Biological Mechanisms: By studying the downstream effects of LRP6 knockout, scientists can uncover the broader biological mechanisms and cellular responses involved. This helps elucidate how cells compensate for the loss of LRP6 function and may identify other key pathways and interactions.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Product updates and innovation

The Human LRP6 Knockout Cell Line-HeLa benefits from regular updates and innovations, ensuring that it remains at the forefront of scientific research and providing researchers with the latest tools to advance their studies in LRP6-related biology.

Canada

2022-05-16

Advanced technology platform

The technology platform used to develop the Human LRP6 Knockout Cell Line-HeLa is state-of-the-art, employing precise and efficient gene editing techniques, which enhances the reliability and accuracy of the knockout cell line for researchers.

Canada

2023-08-31

Diversity of editing strategies

The Human LRP6 Knockout Cell Line-HeLa offers a diversity of editing strategies, allowing researchers to choose the most suitable method for their specific experimental needs, providing flexibility and versatility in scientific research.

United Kingdom

2021-08-19

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Human LRP6 Knockout Cell Line-HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Product updates and innovation

Advanced technology platform

Diversity of editing strategies

Related Products

Related Services