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Human DNMT1 Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0078

Target Gene :   DNMT1 Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0078
Description HCT116 -DNMT1 (-/-) is a cell line with a homozygous knockout of human DNMT1
Target Gene DNMT1
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name DNMT1 DNA (cytosine-5-)-methyltransferase 1 [ Homo sapiens ]
Gene Symbol DNMT1
Synonyms AIM; DNMT; MCMT; CXXC9; HSN1E
Gene Description DNA (cytosine-5-)-methyltransferase 1
GeneID 1786
Uni ProtID I6L9H2
mRNA Refseq NM_001130823.1
Protein Refseq NP_001124295.1
Chromosome Location 19p13.2
Function DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; RNA binding; methyl-CpG binding; protein binding; transcription factor binding; zinc ion binding;
Pathway Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem; Regulation of retinoblastoma protein, organism-specific biosystem;
MIM 126375
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Successful

Human DNMT1 Knockout Cell Line-HCT116 has saved me so much time and has made my gene editing experiments much more successful. I highly recommend it to any researcher.

United States

Outstanding

Human DNMT1 Knockout Cell Line-HCT116 is simply amazing.It is easy to use and produces excellent results every time, which I am very happy with compared to other products

United States

Efficient and productive

I am so glad I decided to try Human DNMT1 Knockout Cell Line-HCT116. It has made my research more efficient and productive.

France

The best in the market

If you are looking for a reliable and efficient gene knockout reagent, look no further than Human DNMT1 Knockout Cell Line-HCT116. It is the best in the market.

United States

Consistently produced excellent results

I have used Human DNMT1 Knockout Cell Line-HCT116 for several experiments now, and it has consistently produced excellent results. I highly recommend it.

United States

Make research easier

Human DNMT1 Knockout Cell Line-HCT116 has made my gene editing process so much easier. I would definitely recommend it to any researcher looking to streamline their research.

United States

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