Human DKK1 HEK293 Cell Lysate

Name: Human DKK1 HEK293 Cell Lysate
SKU: CLOE-1664
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1664 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1664
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human DKK1 precursor (NP_036374.1) (Met 2-His 266). Purification of target protein (s) from this cell lysate has been verified.
Target Gene	DKK1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	DKK1 dickkopf 1 homolog (Xenopus laevis) [ Homo sapiens ]
Gene Symbol	DKK1
Synonyms	SK; DKK-1
Gene Description	dickkopf homolog 1 (Xenopus laevis)
GeneID	22943
Uni ProtID	I1W660
mRNA Refseq	NM_012242.2
Protein Refseq	NP_036374.1
Chromosome Location	10q11.2
Function	growth factor activity; low-density lipoprotein particle receptor binding; protein binding; receptor antagonist activity; signal transducer activity;
Pathway	Direct p53 effectors, organism-specific biosystem; Presenilin action in Notch and Wnt signaling, organism-specific biosystem; Regulation of Wnt-mediated beta catenin signaling and target gene transcription, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem; Wnt Signaling Pathway NetPath, organism-specific biosystem; Wnt signaling network, organism-specific biosystem; Wnt signaling pathway, organism-specific biosystem;
MIM	605189

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