Human CD44 lentiviral particles

Name: Human CD44 lentiviral particles
SKU: LV00227Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00227Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

Quality Control

Gene Information

Cat. No.	LV00227Z
Description	Human CD44 ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type	Lentiviral particle
Gene	CD44
Species	Human
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	CD44 CD44 molecule (Indian blood group) [ Homo sapiens ]
Gene Symbol	CD44
Synonyms	IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Gene Description	CD44 molecule (Indian blood group)
GeneID	960
Uni ProtID	P16070
mRNA Refseq	NM_000610.3
Protein Refseq	NP_000601.3
Chromosome Location	11p13
Function	collagen binding; hyaluronic acid binding; hyaluronic acid binding; hyalurononglucosaminidase activity; protein binding; contributes_to transmembrane signaling receptor activity;
Pathway	Cytokine Signaling in Immune system, organism-specific biosystem; Disease, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Epstein-Barr virus infection, organism-specific biosystem; Epstein-Barr virus infection, conserved biosystem; Glycosaminoglycan metabolism, organism-specific biosystem;
MIM	107269

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