Human CCL5 HEK293 Cell Lysate

Name: Human CCL5 HEK293 Cell Lysate
SKU: CLOE-1119
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1119 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1119
Introduction	This cell lysate over-expresses the human CCL5 (NP_002976.2) fused with a polyhistidine tag and the human Fractalkine mucin-stalk (Phe 101-Gln 341) at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CCL5
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CCL5 chemokine (C-C motif) ligand 5 [ Homo sapiens ]
Gene Symbol	CCL5
Synonyms	SISd; SCYA5; RANTES; TCP228; D17S136E
Gene Description	chemokine (C-C motif) ligand 5
GeneID	6352
Uni ProtID	D0EI67
mRNA Refseq	NM_002985.2
Protein Refseq	NP_002976.2
Chromosome Location	17q11.2-q12
Function	CCR1 chemokine receptor binding; CCR1 chemokine receptor binding; CCR1 chemokine receptor binding; CCR4 chemokine receptor binding; CCR5 chemokine receptor binding; chemoattractant activity; chemokine activity; chemokine activity; chemokine receptor antagonist activity; chemokine receptor binding; heparin binding; phosphatidylinositol phospholipase C activity; phospholipase activator activity; protein binding; protein homodimerization activity; protein kinase activity; protein self-association; receptor signaling protein tyrosine kinase activator activity;
Pathway	Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
MIM	187011

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