Human CCL2 lentiviral particles

Name: Human CCL2 lentiviral particles
SKU: LV00219Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : LV00219Z

Storage : -80℃ Shipping : Frozen on dry ice

Titer: Size:

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Virus Particles Information

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Gene Information

Cat. No.	LV00219Z
Description	Human CCL2 ORF cDNA is cloned into the lentiviral expression vector for maximal stable delivery. The ready-to-use lentiviruses accelerate experiments investigating exogenous gene overexpression phenotypes and downstream molecular impacts.
Product Type	Lentiviral particle
Gene	CCL2
Species	Human
Titer	Varies lot by lot, for example, ≥110^7 TU/mL, ≥110^8 TU/mL, ≥1*10^9 TU/mL etc.
Size	Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots.
Mycoplasma	Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination.
Purity	Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards.
Sterility	The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities.
Proviral Identity Confirmation	All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert.

Gene Name	CCL2 chemokine (C-C motif) ligand 2 [ Homo sapiens ]
Gene Symbol	CCL2
Synonyms	HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Gene Description	chemokine (C-C motif) ligand 2
Gene ID	6347
Uni Prot ID	P13500
m RNA Refseq	NM_002982.3
Protein Refseq	NP_002973.1
Chromosome Location	17q11.2-q12
Function	CCR2 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity; heparin binding; protein kinase activity; receptor binding;
Pathway	Activation of Genes by ATF4, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem;
MIM	158105

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