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Human BAX Knockout Cell Line-HCT116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0034

Target Gene :   BAX Host Cell :   HCT116

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0034
Description HCT116-BAX (-/-) is a cell line with a homozygous knockout of human BAX
Target Gene BAX
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name BAX BCL2-associated X protein [ Homo sapiens ]
Gene Symbol BAX
Synonyms BCL2L4
Gene Description BCL2-associated X protein
Gene ID 581
Uni Prot ID Q07812
m RNA Refseq NM_138761.3
Protein Refseq NP_620116.1
Chromosome Location 19q13.3-q13.4
Function BH3 domain binding; BH3 domain binding; channel activity; identical protein binding; lipid binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein homodimerization activity;
Pathway Activation, translocation and oligomerization of BAX, organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem;
MIM 600040
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Stable trend

At first I wanted to try this cell line just to simply see the trend, if the trend is right then I will continue to use it, if not then I will not use it again, and this cell line did not disappoint me.

United States

Confidence in transfection results

I have tested the results of this transfection with a number of experiments, not only westernblot, but also cell proliferation assays, and lactate dehydrogenase results are also very stable.

United States

Cheap prices

My fair assessment of this cell line is that it is still relatively good, inexpensive and stable for transfection.

United States

Good transfection results

One thing that must be mentioned about this cell line is the amount used, which is not high per use, but the transfection results are good.

United States

Clear result

I used this company for my previous experiments with interfering fragments, and the results were still clear enough for me to trust it, so I ordered the transfection cell line.

United States

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