Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0119
Host Cell : HT29 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0119 |
| Description | This HT-29 cell line was established in 1964 from the primary tumor of a 44-year-old Caucasian female with colorectal adenocarcinoma. The GFP Stable Cell Line-HT-29 constitutively expresses GFP. |
| Host Cell | HT29 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Recommended Medium | Inquiry for instruction of culturing |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
HT-29 is a human colon cancer cell line widely used in biological and cancer research. HT-29 cells, originally extracted from a 44-year-old white woman in 1964 by Jorgen Fogh, form a compact monolayer while displaying similarities to the intestinal epithelial cells of the small intestine. In preclinical studies, HT-29 cells were studied for their ability to differentiate to mimic real colon tissue in vitro, a property that makes HT-29 useful for epithelial cell studies. This cell line is a suitable transfection host and has applications in cancer and toxicology research.
Green fluorescent protein (GFP) is a versatile biomarker used to monitor physiological processes, visualize protein localization, and detect transgene expression in vivo. When excited by blue light (maximum wavelength 395 nm), it can emit green light (maximum wavelength 509 nm). By combining the advantages of GFP and HT-29 cell lines, GFP stable cell line-HT-29 provides researchers with a valuable tool. It can be used in bioimaging studies, studying the cellular behavior of cancer cells, and testing the effectiveness of drugs against colorectal cancer.
GFP stable cell line-HT-29 is mainly used in advanced biological research and medical experiments.
1) Protein iocalization: Using a GFP stable cell line - HT29 can help researchers track the location of specific proteins within cells. By linking the GFP gene to another gene of interest, researchers can visually observe the protein produced by that gene in living cells.
2) Gene expression studies: This cell line is also used to study gene expression because they express a GFP reporter gene that fluoresces when the gene of interest is expressed. This allows scientists to easily monitor gene expression levels.
3) Drug discovery: In drug research, it is used to screen potential drugs for the treatment of gastrointestinal diseases such as colon cancer. Due to its high transfection efficiency, it can help researchers identify potential therapeutic drugs by observing changes in GFP fluorescence intensity.
4) Cell-based detection: GFP stable cell line - HT29 can be used for various cell-based detection, such as cell proliferation, cell migration and cytotoxicity detection, because the fluorescence of GFP is easier to detect compared with traditional colorimetric assays.
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The high consistency of GFP expression across batches has greatly increased the reliability of our experiments. We no longer have to worry about variations in results, which has greatly simplified our workflow.
We are impressed by the strong and stable GFP signal exhibited by the HT-29 cell line. The high fluorescence intensity enhances our imaging studies and makes it easier for us to clearly observe cellular processes in real time.
The HT-29 cell line with GFP we purchased maintained excellent viability and proliferation rates even after several passages. This has been particularly beneficial for our long-term studies.
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