Cat.No. : CSC-RR0119 Host Cell: HT-29
| Cat. No. | CSC-RR0119 |
| Description | This HT-29 cell line was established in 1964 from the primary tumor of a 44-year-old Caucasian female with colorectal adenocarcinoma. The GFP Stable Cell Line-HT-29 constitutively expresses GFP. |
| Host Cell | HT-29 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Recommended Medium | Inquiry for instruction of culturing |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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The high consistency of GFP expression across batches has greatly increased the reliability of our experiments. We no longer have to worry about variations in results, which has greatly simplified our workflow.
We are impressed by the strong and stable GFP signal exhibited by the HT-29 cell line. The high fluorescence intensity enhances our imaging studies and makes it easier for us to clearly observe cellular processes in real time.
The HT-29 cell line with GFP we purchased maintained excellent viability and proliferation rates even after several passages. This has been particularly beneficial for our long-term studies.
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