A549 was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male. In nature, these cells are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across the alveoli of lungs. If A549 cells are cultured in vitro, they grow as monolayer cells, adherent or attaching to the culture flask. The human alveolar epithelial cell line A549 may be anchored or suspended in a solution in vitro. Another characteristic of A549 cells are, they able to synthesize lecithin and contain high level of desaturated fatty acids, which are important to maintain the membrane phospholipids in cells. The GFP Stable Cell Line-A549 constitutively expresses GFP.
This cryovial contains at least 1.0 × 10^6 cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The cells are tested free of microbial contamination.