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FADD Knockout Cell Line-32D

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RT0127

Target Gene :   Fadd Host Cell :   32D

Size :   >1x106 cells/vial Validation :   Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RT0127
Description 32D-FADD cell line is a stable cell line with a homozygous knockout of FADD
Target Gene Fadd
Host Cell 32D
Host Cell Species Mus musculus (Mouse)
Size >1x106 cells/vial
Validation Sequencing
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene FADD
Background The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
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Q & A

Customer Reviews

Customer Q&As
How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews
Accessible

FADD Knockout Cell Line-32D has simplified the complex process of gene editing, making it accessible to researchers from diverse backgrounds and expertise levels.

United States

Effectiveness

I have witnessed significant advancements in my research thanks to the efficiency and effectiveness of FADD Knockout Cell Line-32D.

United States

Reproducible results

The exceptional knockout efficiency of FADD Knockout Cell Line-32D has enabled me to achieve reliable and reproducible results, instilling confidence in the validity of my findings.

United States

High performance

The easy to use nature of FADD Knockout Cell Line-32D, combined with its high performance, has made it an indispensable tool in my laboratory.

United States

Leader

The exceptional quality and performance of FADD Knockout Cell Line-32D justify its reputation as a leader in the field of gene editing.

United States

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