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Cat.No. : CSC-RT0127
Host Cell: 32D Target Gene: Fadd
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0127 |
| Description | 32D-FADD cell line is a stable cell line with a homozygous knockout of FADD |
| Target Gene | Fadd |
| Host Cell | 32D |
| Host Cell Species | Mus musculus (Mouse) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
FADD Knockout Cell Line-32D has simplified the complex process of gene editing, making it accessible to researchers from diverse backgrounds and expertise levels.
United States
07/19/2022
I have witnessed significant advancements in my research thanks to the efficiency and effectiveness of FADD Knockout Cell Line-32D.
United States
11/03/2022
The exceptional knockout efficiency of FADD Knockout Cell Line-32D has enabled me to achieve reliable and reproducible results, instilling confidence in the validity of my findings.
United States
02/23/2023
The easy to use nature of FADD Knockout Cell Line-32D, combined with its high performance, has made it an indispensable tool in my laboratory.
United States
04/11/2023
The exceptional quality and performance of FADD Knockout Cell Line-32D justify its reputation as a leader in the field of gene editing.
United States
08/23/2020
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