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Cat.No. : CSC-RT0121
Host Cell: SV40/MES-13 Target Gene: Fadd
Size: 1x10^6 cells/vial, 1mL Validation: Sequencing
| Cat. No. | CSC-RT0121 |
| Description | SV40/MES-13-FADD cell line is a stable cell line with a homozygous knockout of FADD |
| Target Gene | Fadd |
| Host Cell | SV40/MES-13 |
| Host Cell Species | Mus musculus (Mouse) |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.
A: Single clonal cell.
A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.
A: The cell line should be stored in liquid nitrogen for long-term preservation.
A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Using FADD Knockout Cell Line-SV40/MES-13 was a good tool for my experiments, allowing me to achieve precise and reliable gene editing.
United States
07/19/2022
The high efficiency of FADD Knockout Cell Line-SV40/MES-13 saved me time and resources, resulting in faster progress in my research.
United States
11/03/2022
FADD Knockout Cell Line-SV40/MES-13's performance far exceeded my expectations, making it an indispensable tool in my genetic studies.
United States
02/23/2023
I was highly impressed by the consistent and robust results obtained with FADD Knockout Cell Line-SV40/MES-13, proving its effectiveness.
United States
04/11/2023
FADD Knockout Cell Line-SV40/MES-13 has simplified the process of gene editing, making it accessible to researchers of all experience levels.
United Kingdom
05/09/2023
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