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EpiPro Fast DNA Methylation Kit

Cat. No
DMK-Z03
Description
The Fast DNA Methylation Kit is a complete set of optimized buffers and reagents to perform DNA modification using a next generation DNA bisulfite conversion technology. Through a proprietary composition which allows DNA denaturation and bisulfite conversion to be processed at the same time. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil.
Principle
As a next generation bisulfite conversion tool, the Fast DNA Methylation Kit contains all reagents required for an ultra-fast bisulfite conversion on a DNA sample. With the ready-to-use conversion mix solution, DNA denaturation status is sustained throughout the entire bisulfite DNA conversion process. This proprietary solution allows the bisulfite reagents to rapidly convert all cytosine to uracil with negligible methylcytosine conversion. The unique DNA protection reagents contained in the mix can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic DNA capture solution enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual bisulfite and salts.
Sample Type
DNA
Processing Time
<1.5h
Conversion Efficiency
99.9%
Final Yield
>75%
Vessel Format
Spin Column
Elution Volume
10-20 μl
Storage
Room temperature (15-22 centigrade) away from light
Shipping Condition
The kit is shipped at ambient temperature.
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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