Transfected Stable Cell Lines
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Cat. No. : DMK-Z03
| Cat. No. | DMK-Z03 |
| Description | The Fast DNA Methylation Kit is a complete set of optimized buffers and reagents to perform DNA modification using a next generation DNA bisulfite conversion technology. Through a proprietary composition which allows DNA denaturation and bisulfite conversion to be processed at the same time. Furthermore, it prevents more than 90% of DNA loss, completely converting unmethylated cytosine into uracil. |
| Sample Type | DNA |
| Report Gene | High Efficiency,Fast |
| Efficiency | 99.9% |
| Elution Volume | 10-20 μl |
| Manipulation | <1.5h |
| Selection Marker | NA |
| Yield | >75% |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
| Note | As a next generation bisulfite conversion tool, the Fast DNA Methylation Kit contains all reagents required for an ultra-fast bisulfite conversion on a DNA sample. With the ready-to-use conversion mix solution, DNA denaturation status is sustained throughout the entire bisulfite DNA conversion process. This proprietary solution allows the bisulfite reagents to rapidly convert all cytosine to uracil with negligible methylcytosine conversion. The unique DNA protection reagents contained in the mix can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic DNA capture solution enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual bisulfite and salts. |
| Storage | Room temperature (15-22 centigrade) away from light |
| Shipping | The kit is shipped at ambient temperature. |
A: Depending on the DNA methylation site in the locus, it can serve as a repressive or activating mark for gene expression. In crosstalk with histone modifiers, DNA methylation can thereby inhibit binding of the transcription machinery or create an environment favorable for transcription.
A: DNA methylation analysis is a method used to determine the extent and pattern of DNA methylation at specific regions of the genome.
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This EpiPro Fast DNA Methylation Kit minimizes DNA degradation, reagent preparation, and conversion time.
Ready-to-use conversion reagent is added directly to DNA. Good experimental results were obtained.
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