Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : DMK-Z01
| Cat. No. | DMK-Z01 |
| Description | The DNA Methylation Kit is a complete set of optimized reagents designed to convert DNA so that 5-methylcytosines can be detected in downstream applications, for the purpose of gene-specific DNA methylation analysis. |
| Sample Type | DNA |
| Report Gene | High Efficiency |
| Efficiency | 99.9% |
| Elution Volume | 8-20 μl |
| Manipulation | <4h |
| Selection Marker | NA |
| Yield | >75% |
| Regulatory Statement | For Research Use Only. Not for use in diagnostic procedures. |
| Note | DNA is chemically denatured to allow the sodium bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent chemical and thermophilic degradation of DNA during the sodium bisulfite treatment process. The included non-toxic, modified DNA capture buffer enables the DNA to bind tightly to the column filter, permitting DNA cleaning to be carried out on the column to effectively remove residual sodium bisulfite and salts. |
| Storage | Room temperature (15-25 centigrade) |
| Shipping | Room temperature (15-25 centigrade) |
A: DNA methylation is a process by which a methyl group (CH3) is added to the DNA molecule. This is typically achieved by the addition of a methyl group to the cytosine base of a DNA strand, resulting in the formation of 5-methylcytosine.
A: Altered DNA methylation patterns have been associated with various diseases, including cancer, neurological disorders, and autoimmune conditions.
A: DNA methylation analysis can be performed using various techniques, including bisulfite sequencing, methylation-specific PCR (MSP), methylation-sensitive restriction enzyme digestion, and microarray-based methods.
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Great! The DNA methylation kit offers a fast, efficient and convenient alternative to traditional bisulfite conversions.
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