Cat.No. : CSC-RR0528 Host Cell: B16F10
| Cat. No. | CSC-RR0528 |
| Description | This cell line is engineered to stably overexpress ZsYellow1 reporter gene. B16F10 is a murine melanoma cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of B16F10 cells. |
| Gene | ZsYellow1 |
| Host Cell | B16F10 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: ZsYellow1 is a bright yellow fluorescent protein derived from the Discosoma sp. red fluorescent protein DsRed. It is a monomeric protein that exhibits a high extinction coefficient and strong fluorescence, making it a popular choice for tagging proteins in live cell imaging applications.
A: ZsYellow1's bright yellow fluorescence allows for easy visualization of tagged proteins in live cells, enabling researchers to study protein localization, dynamics, and interactions with high contrast and minimal interference. Its spectral properties make it compatible with various microscopy techniques and dyes, expanding its utility in cell imaging experiments.
A: ZsYellow1 offers several advantages over other yellow fluorescent proteins, such as its high quantum yield, narrow emission spectrum, and fast maturation rate. These properties result in superior sensitivity, less photobleaching, and faster image acquisition, which are particularly beneficial for studying fast-moving proteins or dynamic processes in living cells.
A: While ZsYellow1 is a powerful tool for cell imaging, it does have some limitations. Its bright fluorescence can sometimes lead to high background signals, which may affect the detection of weakly expressing proteins or in situations where the cellular environment is highly autofluorescent. Careful optimization of imaging conditions and the use of spectral filtering can help mitigate these challenges.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
ZsYellow1 reporter cell line - B16F10 changed the game with its intense yellow fluorescence and blew me away! Perfect for my research.
This cell line has powerful luciferase activity and the results are so mind blowing that I am speechless.
The ZsYellow1 Reporter cell line - B16F10 is a dream to culture, easy and straightforward, making my research a breeze.
This cell line is both safe and brilliant, giving me the confidence to push the boundaries of my research.
The ZsYellow1 Reporter Cell Line - B16F10 is inexpensive and offers unrivalled performance.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
