Wheat CRISPR/Cas9 Genome Editing Kit (Hygromycin)

Name: Wheat CRISPR/Cas9 Genome Editing Kit (Hygromycin)
SKU: CC-10
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-10

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Product Information

Cat. No.	CC-10
Description	The Wheat CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by TaU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9. High activity of sgRNA in rice by using TaU6 (wheat U6) promoter. Mutiplex genome editing
Applications	The Wheat CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in wheat.
Resistance	Kanamycin
Selection Marker	Hygromycin B

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What are the characteristics of wheat genome?

A: Bread wheat (Triticum aestivum L.) which is an important crop providing more than 20% of daily calorie intake for humans has a complex genome structure formed with the combination of three different genomes: A, B, and D. The hexaploid nature of the wheat genome makes this plant an important model for studying and optimizing the genome editing system.

What are the difficulties in editing the wheat genome?

A: The polyploid nature of the wheat increases the possibility of off-target mutations and decreases genome editing specificity. Additionally, the editing of each copy of a gene inside the genome is another controversial issue, particularly for genes that possess a high copy number in several genomic locations.

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Customer Reviews

Useful Tool

Great buy! Wheat CRISPR/Cas9 Genome Editing Kit is a useful tool for targeted editing of the wheat genome.

United States

2022-12-19

Great Kit

Great kit. I successfully used the Wheat CRISPR/Cas9 Genome Editing Kit to knockout GASR7 which is a gibberellin-regulated gene that controls grain length in rice.

United States

2023-01-22

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Wheat CRISPR/Cas9 Genome Editing Kit (Hygromycin)

Product Information

Q & A

Customer Reviews

What are the characteristics of wheat genome?

What are the difficulties in editing the wheat genome?

Useful Tool

Great Kit

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