Rice CRISPR/Cas9 Genome Editing Kit (Basta)

Name: Rice CRISPR/Cas9 Genome Editing Kit (Basta)
SKU: CC-08
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-08

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Product Information

Cat. No.	CC-08
Description	The Rice CRISPR/Cas9 Genome Editing Kit is a highly effective and universal gene editing kit. It is intended for Agrobacterium-mediated plant transformation. The CRISPR construct contains a sgRNA expression cassette driven by OsU6 promoter, and a codon optimized Cas9 expression cassette driven by ubiquitin promoter. The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Efficiency	Cas9 expression cassette and sgRNA expression cassette are combined into one vector which offers a higher transformation efficiency.
Features	Two functional components are combined into one vector which offers a higher transfection efficiency. High expression level of Cas9 protein in monocots by using maize ubiquitin promoter and monocot codon optimized-Cas9. High activity of sgRNA in rice by using OsU6 (rice U6) promoter. Mutiplex genome editing
Applications	The Rice CRISPR/Cas9 Genome Editing Kit is applicable to modify genes in almost all kinds of monocots, especially in rice.
Resistance	Kanamycin
Selection Marker	Basta

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Customer Q&As

What is the promoter used for sgRNA (single guide RNA) expression?

A: The CRISPR construct contains a sgRNA expression cassette driven by OsU6 promoter.

What type of Cas9 is used in the CRISPR Plant vectors?

A: Type IIA Cas9 derived from Streptococcus pyogenes.

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Customer Reviews

Reliable

The CRISPR-Cas9 system could offer breeders the potential to engineer high yielding varieties to meet local preferences for stickiness by artificially mimicking natural variation.

United States

2022-12-19

Worked Very Well

The Rice CRISPR/Cas9 Genome Editing Kit helped us successfully introduce non-native genes into the rice genome.

United States

2022-12-20

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Rice CRISPR/Cas9 Genome Editing Kit (Basta)

Product Information

Q & A

Customer Reviews

What is the promoter used for sgRNA (single guide RNA) expression?

What type of Cas9 is used in the CRISPR Plant vectors?

Reliable

Worked Very Well

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