Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VET1284
| Cat. No. | VET1284 |
| Background | The ldhL promoter was amplified by PCR from genomic DNA of L. acidophilus ATCC4356T using oligonucleotides LAldh4 and LAldh3. The first contains the HindIII site and the second contains the EcoRI site and the ldhL ribosome binding site (RBS). The 290 bp PCR product was cloned into pBSGFP3, generating pBS-ldhGFP. ldhGFP was then excised from pBS-ldhGFP via SalI and BamHI digestion and inserted into pTRKH3, generating pTRKH3-ldhGFP. |
| Host Cell | Lactobacillus, Escherichia coli |
| Resistance | Erythromycin |
| Vector Type | Expression Vector |
| Vector Size | 8509 bp |
pTRKH3-ldhGFP is a molecular biology tool widely used in various applications.
Firstly, it is commonly utilized for gene expression studies. Researchers can insert their gene of interest into the pTRKH3-ldhGFP vector to investigate the expression pattern and regulation of the gene in different cell types or organisms. The green fluorescent protein (GFP) tag in the vector allows for easy visualization and localization of the expressed protein under a fluorescence microscope.
Secondly, pTRKH3-ldhGFP can be employed in protein-protein interaction studies. By fusing the target protein with GFP, researchers can investigate its interaction with other proteins of interest.
Additionally, pTRKH3-ldhGFP can be utilized in transgenic animal models to study gene function and regulation. The vector can be used to generate transgenic animals expressing the GFP-tagged protein, enabling non-invasive live imaging of protein expression and localization in various tissues and organs.
In summary, pTRKH3-ldhGFP is a versatile tool widely used for gene expression studies, protein-protein interaction analysis, cell sorting, and transgenic animal research.
A: The pTRKH3-ldhGFP vector has mgfp5 as the selection marker.
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The ldhGFP gene can encode a green fluorescent protein, causing the transformed Lactobacillus strain to emit a bright green fluorescent signal under laser irradiation, allowing researchers to quickly screen and locate the strain.
Whether it is basic research in the laboratory or strain modification in industrial production, pTRKH3-ldhGFP has broad application prospects and can bring convenience to various research and applications.
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