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TNFαPro-Luc/EF1α-mCherry Reporter Cell Line - NIH3T3

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01139

Host Cell :   NIH3T3 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01139
Description TNFαPro-Luc/EF1α-mCherry Reporter Cell Line - NIH3T3
Product Type Signaling pathway reporter cell line
Target Gene TNFαPro-Luc/EF1α-mCherry
Host Cell NIH3T3
Host Cell Species Mus musculus (Mouse)
Applications Signaling pathway studies and screening assay
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Customer Reviews
Ideal for High-Throughput Anti-inflammatory Screening

This TNFα promoter reporter line has been instrumental in our drug discovery pipeline. The sensitivity to TNFα pathway modulators is excellent. We particularly appreciate the stability of the EF1α-mCherry marker, which allows for real-time monitoring of cell health during long-term assays. The quality of the cell line exceeded our expectations.

United States

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