STAT3-Luc/EF1α-mCherry Reporter Cell Line - SGC-7901

STAT3 (Signal Transducer and Activator of Transcription 3) is a key transcription factor in the JAK-STAT signaling cascade and is frequently found in a phosphorylated, constitutively active state in gastric, colorectal, hepatocellular, and several other solid tumors. Its activation is closely associated with tumor cell proliferation, anti-apoptotic signaling, immune evasion, and poor patient prognosis. The STAT3-Luc/EF1α-mCherry Reporter Cell Line - SGC-7901 is built on the SGC-7901 human gastric adenocarcinoma background and carries multiple STAT3 response elements upstream of a firefly luciferase gene, with an mCherry fluorescent marker and a puromycin resistance cassette driven by the constitutive EF1α promoter. Together, this dual-reporter design enables quantitative luminescence readout of STAT3 transcriptional activity while mCherry provides a constitutive fluorescent control for cell number, transfection, or viability normalization.

This reporter line is widely used in oncology and inflammation research focused on the gastric cancer microenvironment. The most common applications include screening JAK family inhibitors (such as AG490, ruxolitinib, fedratinib, and their analogs) for their ability to suppress STAT3 phosphorylation, validating the effects of upstream cytokines like IL-6, IL-10, and EGF on STAT3 activation, and studying downstream STAT3 target genes (such as BCL-2, MCL-1, MYC, CCND1, and EZH2) in gastric cancer models. The system is useful as a readout in drug combination experiments, particularly when combined with NFκB or Myc reporter lines to dissect pathway crosstalk, and in studies exploring how tumor microenvironmental signals (such as TGR5 agonists, bile acids, or inflammatory stimuli) modulate STAT3-dependent transcription. The mCherry control also allows direct visualization of tumor cells in invasion, migration, or co-culture assays, making this line a flexible tool for both biochemical screening and phenotypic studies in gastric cancer biology.