rassf8 Easy KO Kit (Human)

Name: rassf8 Easy KO Kit (Human)
SKU: CC-1609
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CC-1609

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Product Information

Gene Information

Cat. No.	CC-1609
Description	A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr	Rassf8
Gene Name	RASSF8 Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 [ Homo sapiens ]
Species	Human
Ensembl ID	ENSMUSG00000030259
NCBIGene ID	71323
Uni Prot ID	Q8CJ96
Features	All-in-One workflow from gene editing to knockout validation for users with no prior experience. Pre-validated sgRNAs and primers for rapid setup. Streamlined experiment handling. CRISPR RNP method ensures precise and efficient gene knockout.
Applications	This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions	5–10 reactions per target gene
Kit Components	2–3 chemically synthesized sgRNAs (200pmol each) 3 PCR/Sequencing primers (500pmol each) LM cell lysate (500µL) Cas9 protein (12µg) LM RNP transfection reagent (50µL)
Storage	Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.

Gene Name	RASSF8 Ras association (RalGDS/AF-6) domain family (N-terminal) member 8 [ Homo sapiens ]
Gene Symbol	RASSF8
Synonyms	RASSF8; Ras association (RalGDS/AF-6) domain family (N-terminal) member 8; C12orf2, chromosome 12 open reading frame 2; ras association domain-containing protein 8; HoJ 1; carcinoma-associated protein HOJ-1; HOJ1; C12orf2; FLJ11542; DKFZp434O0227;
Gene ID	11228
Uni Prot ID	Q8NHQ8
m RNA Refseq	BC030021
Chromosome Location	12p12.3
MIM	608231

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