p53-Luc/EF1α-mCherry Reporter Cell Line - U87MG

The p53-Luc/EF1α-mCherry reporter cell line (based on U87MG) is a genetically engineered human glioblastoma model derived from the U87MG cell line. It incorporates a firefly luciferase reporter gene driven by a p53-responsive promoter, enabling the quantitative monitoring of transcriptional activity regulated by the p53 signaling pathway. Additionally, the mCherry fluorescent protein is constitutively expressed under the control of the EF1α promoter, providing a stable fluorescent marker for the identification and tracking of these reporter cells. This dual-reporter system combines an inducible luminescent signal with a continuous red fluorescent signal, allowing researchers to assess p53 pathway activation while simultaneously observing cell distribution, morphology, and viability.

The p53-Luc/EF1α-mCherry reporter cell line (based on U87MG) is well-suited for investigating p53-mediated responses within the context of glioblastoma biology and anticancer drug development. It can be utilized to screen for small-molecule compounds, biologics, and combination therapies that activate or inhibit p53-dependent transcription. The luciferase signal intensity serves as a sensitive and quantifiable readout for dose-response studies, high-throughput screening, and the analysis of pathway regulation during mechanism-of-action studies. The constitutively expressed mCherry signal facilitates the normalization of experimental data and supports applications such as live-cell imaging, cell counting, transduction monitoring, and quality control. Furthermore, this model can be applied to research concerning DNA damage, cellular stress, apoptosis, therapeutic resistance, and gene regulation. By integrating a pathway-specific reporter system with a stable fluorescent reference, this cell line enhances experimental consistency and provides a practical platform for translational research focused on the p53 signaling pathway in glioblastoma.