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p53-Luc/EF1α-mCherry Reporter Cell Line - HCT 116

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01107

Host Cell :   HCT116 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01107
Description p53-Luc/EF1α-mCherry Reporter Cell Line - HCT 116
Product Type Signaling pathway reporter cell line
Target Gene p53-Luc/EF1α-mCherry
Host Cell HCT116
Host Cell Species Homo sapiens (Human)
Applications Signaling pathway studies and screening assay
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Work well

We utilized this cell line to study the efficacy of DNA-damaging agents in colorectal cancer. The luciferase readout correlates perfectly with p53 protein levels we see in Western blots. The cells arrived healthy and grew rapidly, making it easy to scale up for our experiments.

United States

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