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CC-978

NSUN3 Easy KO Kit (Mouse)

For research use only. Not intended for any clinical use.

Cat. No. :   CC-978

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Product Information

Gene Information

Cat. No. CC-978
Description A complete kit for efficient gene knockout in mammalian cells, combining chemically synthesized sgRNAs with Cas9 RNPs to induce targeted DNA cleavage and generate frameshift mutations or deletions. All essential reagents for transfection and knockout validation are included for rapid, high-efficiency gene disruption.
Gene Abbr Nsun3
Gene Name Nsun3 NOL1/NOP2/Sun domain family member 3 [ Mus musculus ]
Species Mouse
EnsemblID ENSMUSG00000050312
NCBIGeneID 106338
Uni ProtID Q8CCT7
Features
  • All-in-One workflow from gene editing to knockout validation for users with no prior experience.
  • Pre-validated sgRNAs and primers for rapid setup.
  • Streamlined experiment handling.
  • CRISPR RNP method ensures precise and efficient gene knockout.
Applications This kit enables in vitro gene knockout in human-derived cells using chemically synthesized sgRNAs and Cas9-gRNA RNP complexes. Transfected RNPs cleave early exons of the target gene, inducing deletions or frameshift mutations for efficient and rapid knockout.
Reactions 5–10 reactions per target gene
Kit Components 2–3 chemically synthesized sgRNAs (200pmol each)
3 PCR/Sequencing primers (500pmol each)
LM cell lysate (500µL)
Cas9 protein (12µg)
LM RNP transfection reagent (50µL)
Storage Store at -80°C for up to 1 year or at -20°C for up to 6 months. Avoid repeated freeze-thaw cycles.
Gene Name Nsun3 NOL1/NOP2/Sun domain family member 3 [ Mus musculus ]
Gene Symbol Nsun3
Synonyms AU022521; 6720484A09Rik
Gene Description NOL1/NOP2/Sun domain family 3
GeneID 106338
Uni ProtID Q8CCT7
mRNA Refseq NM_178925.3
Protein Refseq NP_849256.1
Chromosome Location 16 C1.3; 16
Function methyltransferase activity; molecular_function; transferase activity;
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