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Stable expression over 15 generations with rapid cell line development in just 3 months.
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Cat.No. : CSC-RR01242 Host Cell: U2932
| Cat. No. | CSC-RR01242 |
| Description | This cell line is engineered to stably exprress NanoLuc Luciferase(NLuc) reporter gene in U2932 cells. It is a useful tool for bioluminescent tracking of U2932 cells. |
| Gene | Nluc |
| Host Cell | U2932 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Bioluminescent Reporter Cell Lines |
| Applications | in vitro cell tracking and in vivo cell imaging |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Suspension cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Working with suspension lymphoma lines can sometimes be tricky regarding transfection, so buying this stable U2932 Nluc line was a great decision. The Nluc signal is strong, and the cells proliferate well. It has greatly simplified our workflow for monitoring tumor progression in lymph node models
Germany
06/20/2024
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