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Nluc Reporter Cell Line - U2932

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01242

Host Cell :   U-2932 Size :   >1x106 frozen cells/vial

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Cell Culture Information

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Cat. No. CSC-RR01242
Description This cell line is engineered to stably exprress NanoLuc Luciferase(NLuc) reporter gene in U2932 cells. It is a useful tool for bioluminescent tracking of U2932 cells.
Product Type Bioluminescent Reporter Cell Lines
Target Gene Nluc
Host Cell U-2932
Host Cell Species Homo sapiens (Human)
Applications in vitro cell tracking and in vivo cell imaging
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Suspension cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Great Performance

Working with suspension lymphoma lines can sometimes be tricky regarding transfection, so buying this stable U2932 Nluc line was a great decision. The Nluc signal is strong, and the cells proliferate well. It has greatly simplified our workflow for monitoring tumor progression in lymph node models

United States

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