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Nluc Reporter Cell Line - RENCA

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01231

Host Cell :   Renca Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01231
Description This cell line is engineered to stably exprress NanoLuc Luciferase(NLuc) reporter gene in RENCA cells. It is a useful tool for bioluminescent tracking of RENCA cells.
Product Type Bioluminescent Reporter Cell Lines
Target Gene Nluc
Host Cell Renca
Host Cell Species Mus musculus (Mouse)
Applications in vitro cell tracking and in vivo cell imaging
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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Customer Reviews
Perfect for syngeneic renal cancer models

For our immunotherapy research requiring immunocompetent hosts, this RENCA Nluc line was exactly what we needed. The Nluc tag did not appear to alter the immunogenicity or growth rate of the cells. The brightness of the reporter made detecting lung metastases much easier than with previous lines we have tried.

Germany

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