Nluc Reporter Cell Line - H22

The H22 cell line is a mouse hepatocarcinoma cell line, originally derived from a mouse hepatocellular carcinoma. Due to its relevance in studying liver cancer biology, tumor progression, and treatment response, this cell line has been widely used in cancer research. Hepatocellular carcinoma (HCC) is one of the most common and aggressive types of liver cancer, and the H22 cell line provides a valuable model for studying the molecular mechanisms of tumor growth, metastasis, and drug resistance. Researchers have utilized H22 cells in various experimental settings, including in vitro experiments and in vivo mouse models, to evaluate the efficacy of anticancer drugs, understand immune responses, and explore new therapeutic strategies.

The Nluc reporter gene cell line - H22 is an engineered version of the H22 cell line that incorporates the NanoLuc (Nluc) luciferase reporter gene. This modification allows for highly sensitive and quantitative detection of cellular activity through bioluminescence imaging. This reporter gene cell line is versatile and powerful, particularly in the fields of oncology and drug development. For example, it can be used to monitor tumor growth, metastasis, and response to therapeutic interventions in real-time in living animal models, allowing researchers to track disease progression in a non-invasive manner. The high signal-to-noise ratio of NanoLuc luciferase ensures accurate measurements, making it ideal for high-throughput screening of anticancer compounds. Furthermore, this reporter gene cell line facilitates the study of tumor microenvironment interactions, immune cell infiltration, and the effectiveness of immunotherapies.