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Cat.No. : CSC-RR01189 Host Cell: E0771
| Cat. No. | CSC-RR01189 |
| Description | This cell line is engineered to stably exprress NanoLuc Luciferase(NLuc) reporter gene in E0771 cells. It is a useful tool for bioluminescent tracking of E0771 cells. |
| Gene | Nluc |
| Host Cell | E0771 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Bioluminescent Reporter Cell Lines |
| Applications | in vitro cell tracking and in vivo cell imaging |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent cell line |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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E0771 Nluc cells yielded strong, low-background luminescence in vitro and translated well to pilot in vivo work. Viability post-thaw was high, and reporter expression remained stable without drift across passages.
United Kingdom
03/24/2024
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