NFκB-Luc/EF1α-mCherry Reporter Cell Line - U87MG

NFκB-Luc/EF1α-mCherry Reporter Cell Line - U87MG is a genetically engineered human glioblastoma cell line derived from the parental U87MG model. The cells contain a luciferase reporter gene controlled by an NFκB-responsive promoter, allowing activation of the NFκB signaling pathway to be quantified through luminescence. They also stably express mCherry under the EF1α promoter, providing a constitutive red fluorescent signal for visual identification, cell tracking, and normalization of cell number or viability. This dual-reporter design combines pathway-specific bioluminescence with stable fluorescence, making the cell line suitable for reproducible, real-time analysis of NFκB activity in a glioblastoma-relevant cellular background.

The reporter cell line is primarily used to evaluate compounds, biologics, genetic interventions, and cellular stimuli that regulate NFκB signaling. It can support high-throughput screening for NFκB inhibitors or activators, mechanism-of-action studies, and investigations of inflammatory, immune, stress, and survival pathways in glioblastoma cells. The luciferase readout provides a sensitive, quantitative measure of transcriptional activity, while mCherry fluorescence enables routine culture monitoring, imaging, cell counting, and quality control. Researchers may apply the model to assess responses to cytokines such as TNF-α or IL-1β, examine pathway modulation induced by pharmacological treatments, and compare gene knockdown or overexpression conditions. It is also useful for studying tumor–immune interactions, screening anti-inflammatory or anticancer candidates, and establishing co-culture or drug-response assays.