NFκB-Luc/EF1α-mCherry Reporter Cell Line - HEK293

NFκB-Luc/EF1α-mCherry Reporter Cell Line - HEK293 is a genetically engineered human embryonic kidney 293 cell line designed for sensitive monitoring of nuclear factor kappa B (NF-κB) pathway activity. The cells stably express a firefly luciferase reporter driven by NF-κB-responsive elements, allowing pathway activation to be quantified through luminescence measurement. In parallel, mCherry is constitutively expressed under the human elongation factor 1 alpha (EF1α) promoter, providing a robust fluorescent marker for cell identification, normalization, and assessment of transfection or treatment-related effects on cell number. NF-κB is a central transcription factor involved in inflammatory signaling, innate and adaptive immune responses, cellular stress, proliferation, and survival. In resting cells, NF-κB is retained in the cytoplasm by inhibitory IκB proteins. Stimulation by cytokines, pathogen-associated molecules, oxidative stress, or other activators promotes IκB degradation and NF-κB nuclear translocation, resulting in transcriptional activation of NF-κB target genes and luciferase expression in this reporter system.

This reporter cell line is designed for the cellular screening and mechanistic study of modulators of the NF-κB signaling pathway. It can be used to evaluate a wide range of substances that regulate NF-κB pathway activity, including anti-inflammatory compounds, immune agonists, kinase inhibitors, biologics, natural products, and therapeutic drug candidates. Typical applications include assessing cellular responses to TNF-α, IL-1β, lipopolysaccharide (LPS), Toll-like receptor (TLR) agonists, and other inflammatory stimuli, as well as determining dose-response relationships, potency, efficacy, and pathway selectivity. The luciferase-based detection method enables rapid, quantitative, and scalable assays in a multi-well plate format, making it well-suited for high-throughput or medium-throughput drug discovery workflows. Furthermore, this cell line can be utilized in research areas such as inflammation, cancer biology, infection, stress responses, and signal transduction—fields in which NF-κB activity is frequently employed as a key functional endpoint.