Myc-Luc/EF1α-mCherry Reporter Cell Line - SH-SY5Y

Myc-Luc/EF1α-mCherry Reporter Cell Line - SH-SY5Y is a human neuroblastoma-derived reporter cell line engineered for sensitive monitoring of MYC transcriptional activity. The cells constitutively express mCherry under the control of the human EF1α promoter, providing a stable fluorescent reference for cell identification, transduction assessment, and normalization of cell number or viability. A luciferase reporter linked to MYC-responsive regulatory elements enables quantitative detection of changes in MYC pathway activity through luminescence-based assays. SH-SY5Y cells are widely used in neuroblastoma and neuronal biology research because they retain neuronal characteristics and can be differentiated under appropriate culture conditions. The dual-reporter design combines a pathway-responsive luminescent signal with constitutive red fluorescence, supporting robust and convenient analysis in live-cell and endpoint experimental formats.

This reporter cell line is suitable for screening and profiling small molecules, biologics, and genetic perturbations that modulate MYC signaling. It can be used to identify inhibitors of MYC-driven transcription, evaluate dose-response relationships, and characterize the kinetics of pathway activation or suppression following treatment. The EF1α-mCherry signal facilitates normalization for cell abundance, transfection or infection efficiency, and nonspecific cytotoxicity, helping distinguish direct effects on MYC activity from treatment-related loss of viability. Applications include CRISPR-based functional genomics, RNA interference studies, compound screening, target validation, and investigation of resistance mechanisms in MYC-associated neuroblastoma. The model may also support studies of oncogenic signaling crosstalk, neuronal differentiation, and therapeutic combinations involving MYC pathway modulators.