Myc-Luc/EF1α-mCherry Reporter Cell Line - HeLa

The ISRE-Luc/EF1α-mCherry Reporter Cell Line - HeLa is a genetically engineered human cervical cancer cell line designed for the sensitive monitoring of the interferon-stimulated response element (ISRE) signaling pathway. Derived from HeLa cells, this line stably expresses firefly luciferase under the control of an ISRE-containing promoter, enabling the quantitative assessment of Type I interferon activity and the associated JAK-STAT signaling pathway via luminescence detection. Additionally, the cells constitutively express the fluorescent protein mCherry, driven by the human EF1α promoter. This red fluorescent marker facilitates the observation of cell morphology, verification of cell identity, assessment of transduction efficiency, and data normalization in imaging-based experiments. Upon stimulation with interferon-α, interferon-β, or other ISRE-dependent transcriptional activators, luciferase expression is induced and can be measured using standard luciferase assay reagents. This dual-reporter system combines a robust functional readout with continuous fluorescent cell tracking.

The ISRE-Luc/EF1α-mCherry Reporter Cell Line - HeLa is well-suited for research in areas such as innate immunity, antiviral responses, cytokine signaling, and the regulation of interferon pathways. It can be employed to screen small molecules, biologics, nucleic acids, or viral factors to evaluate their ability to activate or inhibit ISRE-mediated transcription. The cell line is particularly effective for assessing inhibitors or enhancers of JAK kinases, STAT proteins, interferon receptors, and their downstream transcriptional regulators. In virology, it provides a practical platform for investigating viral immune-evasion mechanisms and evaluating host cell responses to infection or the expression of viral proteins. The mCherry signal allows for rapid monitoring of cell health and density during compound treatment, while the luciferase signal provides a sensitive, scalable endpoint readout compatible with multi-well plate assay formats. Therefore, this reporter cell line is suitable for both mechanistic studies and high-throughput screening applications.