mScarlet/LUC Reporter Cell Line - NCI-H1975

NCI-H1975 is a human non-small cell lung cancer (NSCLC) cell line established from a lung adenocarcinoma and is widely recognized as a clinically relevant model for studying epidermal growth factor receptor (EGFR)-driven lung cancer. The cell line harbors both the activating EGFR L858R mutation and the EGFR T790M resistance mutation, making it particularly valuable for investigating mechanisms of acquired resistance to EGFR-targeted therapies. NCI-H1975 cells exhibit robust tumorigenic potential and have been extensively used in studies of oncogenic signaling, therapeutic resistance, tumor progression, and precision oncology. The mScarlet/LUC Reporter Cell Line - NCI-H1975 is generated through stable integration of the red fluorescent protein mScarlet and firefly luciferase (LUC) reporter genes into parental NCI-H1975 cells. This dual-reporter system enables highly sensitive fluorescence imaging and quantitative bioluminescence monitoring while maintaining the key molecular and biological characteristics of the original cell line.

The NCI-H1975-mScarlet/LUC reporter cell line is widely utilized in lung cancer research for real-time evaluation of tumor growth, metastatic behavior, and therapeutic efficacy. Luciferase expression enables non-invasive, longitudinal monitoring of tumor burden and treatment response through bioluminescence imaging in xenograft models, providing a sensitive method for assessing disease progression over time. Meanwhile, mScarlet expression offers bright and stable red fluorescence for high-resolution visualization of cellular morphology, proliferation, migration, invasion, and tissue localization. This model is particularly valuable for evaluating EGFR-targeted therapies, next-generation tyrosine kinase inhibitors, combination treatment strategies, and approaches designed to overcome drug resistance in NSCLC. In addition, the dual-reporter platform supports mechanistic studies of EGFR signaling, tumor–microenvironment interactions, epithelial–mesenchymal transition, and metastatic progression. With stable reporter expression and robust imaging performance, the NCI-H1975-mScarlet/LUC cell line serves as a reliable and reproducible tool for preclinical drug discovery and translational research in EGFR-mutant non-small cell lung cancer.