Mouse Tspan4 Stable Cell Line - RAW 264.7

Name: Mouse Tspan4 Stable Cell Line - RAW 264.7
SKU: CSC-RO01370
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO01370

Host Cell : RAW264.7 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO01370
Description	This cell line is engineered to stably express Mus musculus (Murine, House mouse) tetraspanin 4 (Tspan4) in Mouse macrophage cell line (RAW 264.7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type	Mouse gene overexpression stable cell line
Target Gene	Tspan4
Gene Species	Mus musculus (Murine, House mouse)
Host Cell	RAW264.7
Host Cell Species	Mus musculus (Mouse)
Reporter	GFP
Applications	1) investigation of gene function 2) screening and validation of antibodies
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

TSPAN4

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

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