Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01363
Host Cell : RAW264.7 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01363 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) tetraspanin 4 (Tspan4) in Mouse macrophage cell line (RAW 264.7). |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Tspan4 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | RAW264.7 |
| Host Cell Species | Mus musculus (Mouse) |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | TSPAN4 |
| Background | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein and is similar in sequence to its family member CD53 antigen. It is known to complex with integrins and other transmembrane 4 superfamily proteins. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
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The Mouse Tspan4 Stable Cell Line in RAW 264.7 delivered uniform surface expression suitable for flow analysis with minimal drift over time. Packaging and transit were excellent, and the team provided practical advice on culture conditions and gating strategies.
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