Mouse Sgk1 Stable Cell Line - RAW 264.7

Name: Mouse Sgk1 Stable Cell Line - RAW 264.7
SKU: CSC-RO01365

Cat.No. : CSC-RO01365 Host Cell: Mouse macrophage cell line (RAW 264.7)

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Gene Informationn

Cat. No.	CSC-RO01365
Description	This cell line is engineered to stably express Mus musculus (Murine, House mouse) serum/glucocorticoid regulated kinase 1 (Sgk1) in Mouse macrophage cell line (RAW 264.7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Reporter	GFP
Gene	Sgk1
Gene Species	Mus musculus (Murine, House mouse)
Host Cell	Mouse macrophage cell line (RAW 264.7)
Host Cell Species	Mus musculus (Murine, House mouse) cell line
Stability	This cell line is stable at least 10 passages.
Product Type	Mouse gene overexpression stable cell line
Applications	1) investigation of gene function 2) screening and validation of antibodies
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Gene Name	SGK1 serum/glucocorticoid regulated kinase 1 [ Gallus gallus (chicken) ]
Gene Symbol	SGK1
Synonyms	SGK1; serum/glucocorticoid regulated kinase 1; SGK; serine/threonine-protein kinase Sgk1; serum/glucocorticoid-regulated kinase 1; serum- and glucocorticoid-induced kinase;
Gene Description	serum/glucocorticoid regulated kinase 1
Gene ID	395133
UniProt ID	Q6U1I9
mRNA Refseq	NM_204476
Protein Refseq	NP_989807
Chromosome Location	chromosome: 3
Function	ATP binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity;
Pathway	FoxO signaling pathway; Insulin Signaling;

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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The Sgk1 gene encodes serum/glucocorticoid-regulated kinase 1 (SGK1), a protein kinase belonging to the AGC family. SGK1 activity is primarily regulated by cellular stress and hormones via the PI3K signaling pathway. SGK1 is a core regulator of ion transport (especially ENaC) and plays a crucial role in regulating cell volume, survival, and proliferation. Clinically, SGK1 dysregulation is associated with hypertension, diabetes, and fibrosis. In oncology, SGK1 is often overexpressed in drug-resistant tumors, protecting cells from chemotherapy-induced apoptosis and promoting epithelial-mesenchymal transition (EMT).

A stable mouse Sgk1 cell line in the RAW 264.7 background provides a reliable model for studying stress-regulated kinases in macrophage-mediated inflammation. RAW 264.7 is highly sensitive to mineralocorticoid and glucocorticoid signaling. By stably overexpressing mouse Sgk1, this line allows researchers to assess how SGK1 activity affects macrophage responses to osmotic stress and the secretion of pro-fibrotic factors. This product is of great value for screening selective SGK inhibitors, which are expected to become potential drugs for the treatment of fibro-inflammatory diseases and sensitizers for cancer treatment.

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