Mouse Nlrp3 Stable Cell Line - RAW 264.7

Name: Mouse Nlrp3 Stable Cell Line - RAW 264.7
SKU: CSC-RO01372
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO01372

Host Cell : RAW264.7 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO01372
Description	This cell line is engineered to stably express Mus musculus (Murine, House mouse) NLR family, pyrin domain containing 3 (Nlrp3) in Mouse macrophage cell line (RAW 264.7). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type	Mouse gene overexpression stable cell line
Target Gene	Nlrp3
Gene Species	Mus musculus (Murine, House mouse)
Host Cell	RAW264.7
Host Cell Species	Mus musculus (Mouse)
Reporter	GFP
Applications	1) investigation of gene function 2) screening and validation of antibodies
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

NLRP3

Background

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020]

Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Great product!

Creative Biogene's Nlrp3 stable line has simplified our inflammasome activation experiments. The cells show a very clean background with strong inducible responses. It has allowed us to bypass the variability typically associated with transient transfections in RAW 264.7 cells.

United Kingdom

2025-01-14

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-RO0023	hSLC5A5 Overexpression Stable Cell Line-Mel624	Inquiry
CSC-SC000018	Panoply™ Human AAR2 Over-expressing Stable Cell Line	Inquiry
CSC-SC000073	Panoply™ Human ABHD1 Over-expressing Stable Cell Line	Inquiry
CSC-SC000093	Panoply™ Human ABI3BP Over-expressing Stable Cell Line	Inquiry
CSC-SC000060	Panoply™ Human ABCD1 Over-expressing Stable Cell Line	Inquiry
CSC-SC000074	Panoply™ Human ABHD10 Over-expressing Stable Cell Line	Inquiry
CSC-SC000055	Panoply™ Human ABCC4 Over-expressing Stable Cell Line	Inquiry
CSC-SC000075	Panoply™ Human ABHD11 Over-expressing Stable Cell Line	Inquiry
CSC-SC000004	Panoply™ Human A4GALT Over-expressing Stable Cell Line	Inquiry
CSC-SC000058	Panoply™ Human ABCC8 Over-expressing Stable Cell Line	Inquiry

Mouse Nlrp3 Stable Cell Line - RAW 264.7

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Q & A

Customer Reviews

Great product!

Related Products

Related Services