Mouse Irf7 Stable Cell Line - LLC

Name: Mouse Irf7 Stable Cell Line - LLC
SKU: CSC-RO02537
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO02537

Host Cell : LLC Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO02537
Description	This cell line is engineered to stably overexpress Mouse Irf7 in LLC cell line. GFP reporter gene is also expressed in this cell line for cell tracking.
Product Type	Stable cell line constitutively expressing target gene
Target Gene	Irf7
Gene Species	Mouse
Host Cell	LLC
Host Cell Species	Mus musculus (Mouse)
Applications	Gene function studies and others
Size	One vial of frozen cells, typically >1x10^6cells/vial
Stability	This cell line is stable at least 10 passages.
Quality Control	1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry, 2) Mycoplasma detection
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Target Gene

IRF7

Background

This gene encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. It has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. The encoded protein plays an important role in the innate immune response against DNA and RNA viruses. [provided by RefSeq, Jul 2021]

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