mCherry Reporter Cell Line - SGC-7901

The mCherry Reporter SGC-7901 cell line is a robust biological model derived from the well-characterized human gastric adenocarcinoma line, SGC-7901. Originally established from a metastatic lymph node of a patient with gastric cancer, SGC-7901 cells demonstrate an adherent epithelial-like morphology and serve as a cornerstone in oncology research for understanding gastric malignancy. This specific reporter variant has been engineered to achieve stable, constitutive expression of the mCherry red fluorescent protein, typically through high-titer lentiviral transduction followed by rigorous selection for high-expression clones. The resulting cell line emits a powerful, photostable red signal with an excitation maximum at 587 nm and an emission peak at 610 nm. This stable genetic integration allows for the continuous visualization of cell populations across multiple passages without the toxicity associated with transient transfection or the signal decay often seen with chemical dyes.

In terms of practical application, the mCherry SGC-7901 line is exceptionally suited for both high-resolution microscopy and large-scale quantitative assays. In laboratory settings, it facilitates the real-time tracking of cell migration, invasion, and proliferation, as well as the evaluation of drug-induced apoptosis via flow cytometry or high-content screening. Beyond in vitro assays, this reporter line is particularly valuable for in vivo oncology studies. When utilized in subcutaneous or orthotopic xenograft mouse models, the stable fluorescence enables researchers to monitor tumor burden and metastatic spread non-invasively using fluorescence molecular tomography or whole-body imaging systems. This allows for the longitudinal assessment of therapeutic candidates over time in the same animal, significantly increasing the statistical power of preclinical trials. Its versatility makes it an essential tool for investigating the complex mechanisms of tumor biology and validating novel anti-tumor interventions in a physiological context.