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LSL-GFP Reporter Cell Line - HeLa

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RR01062

Host Cell :   HeLa Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RR01062
Description LSL-GFP Reporter Cell Line - HeLa
Product Type Reporter cell line
Target Gene LSL-GFP
Host Cell HeLa
Host Cell Species Homo sapiens (Human)
Applications Evaluation of Cre recombinase activites
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent cell line
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
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High-quality reporter line

We used this LSL-GFP HeLa line to validate our viral Cre vectors. The ‘Stop’ cassette works perfectly—we saw zero background fluorescence in the absence of Cre, and incredibly bright GFP signals immediately after infection.

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