ISRE-Luc/EF1α-mCherry Reporter Cell Line - T24

The ISRE-Luc/EF1α-mCherry Reporter Cell Line - T24 is a stable human bladder carcinoma cell line designed to quantitatively monitor the activation of the JAK-STAT signaling pathway. This dual-reporter system incorporates the Firefly luciferase gene under the transcriptional control of a promoter containing multiple Interferon-Stimulated Response Elements (ISRE). When Type I or Type III interferons bind to their respective receptors, they trigger a signaling cascade that results in the binding of transcription factors to the ISRE, subsequently driving luciferase expression in a concentration-dependent manner. For improved reliability, the cell line constitutively expresses mCherry fluorescent protein under the elongation factor 1-alpha (EF1α) promoter. This internal marker facilitates the normalization of luciferase signals against cell density and serves as a tool for visual verification and selection during experimental procedures.

In the context of biomedical research and drug development, this reporter cell line offers a streamlined platform for investigating innate immune responses and screening therapeutic candidates. It is extensively used in high-throughput assays to evaluate the potency of interferon-based drugs and to identify small molecules that modulate the JAK-STAT pathway, which is central to antiviral defense and tumor immunology. Beyond drug screening, the T24 reporter line is effective for studying viral interference with host signaling and for assessing the bioactivity of cytokines in complex biological matrices. Its stable expression profile and robust bioluminescent output make it an essential tool for characterizing the efficacy of anti-inflammatory compounds and validating the molecular targets of novel immunotherapies within an epithelial cell background.