ISRE-Luc/EF1α-mCherry Reporter Cell Line - A549

ISRE-Luc/EF1α-mCherry Reporter Cell Line - A549 is a human lung adenocarcinoma-derived reporter cell line engineered for sensitive monitoring of interferon-stimulated response element (ISRE) activity. The parental A549 cell line provides a widely used pulmonary epithelial model with intact innate immune signaling pathways, making it suitable for studies of antiviral and interferon responses. In this dual-reporter system, firefly luciferase expression is driven by an ISRE-containing promoter, enabling quantitative measurement of transcriptional activation downstream of type I interferon signaling and related pathways. In parallel, mCherry is constitutively expressed under the human EF1α promoter, serving as a stable fluorescent marker for cell identification, normalization, imaging, and monitoring of cell number or viability. Following stimulation with interferons, viral mimetics, or other innate immune activators, activation of the JAK-STAT pathway induces ISRE-dependent luciferase expression, producing a measurable luminescent signal.

This reporter cell line is particularly valuable for screening compounds that modulate interferon signaling, innate immunity, and antiviral defense. It can be used to evaluate the activity of recombinant interferons, cytokines, small molecules, nucleic acid-based therapeutics, and biological agents that enhance or suppress ISRE-mediated transcription. The model is also applicable to antiviral drug discovery, allowing researchers to investigate how viral infection or viral proteins influence host interferon responses. In high-content imaging workflows, constitutive mCherry fluorescence facilitates rapid visualization of reporter cells, assessment of transduction efficiency, and normalization across treatment conditions. The cell line can further support mechanistic studies of JAK-STAT signaling, interferon receptor function, pattern-recognition receptor pathways, and immune evasion mechanisms.