IL6pro-Luc/EF1α-mCherry Reporter Cell Line - U87MG

The IL6pro-Luc/EF1α-mCherry Reporter Cell Line - U87MG is a stably engineered human glioblastoma cell model based on the U87MG cell line. It contains a luciferase reporter gene driven by the promoter region of the human interleukin-6 (IL6) gene, enabling quantitative monitoring of IL6 promoter activity through bioluminescence. In addition, an EF1α-driven mCherry fluorescent marker is incorporated to facilitate the identification, visualization, and tracking of reporter cells during routine culture and experimental procedures. U87MG cells are widely used in studies of glioblastoma biology, neuroinflammation, tumor signaling, and drug response. The dual-reporter design provides both a sensitive functional readout and a convenient fluorescence-based method for cell localization and quality control.

This reporter cell line is suitable for investigating the regulation of IL6 transcription in response to inflammatory stimuli, cytokines, stress signals, genetic manipulation, or candidate compounds. Luciferase activity can be measured with a luminometer to provide a sensitive, scalable, and quantitative assessment of IL6 promoter activation, making the model useful for pathway analysis, mechanism-of-action studies, and high-throughput screening. The mCherry signal can be used to monitor cell morphology, estimate reporter-cell abundance, evaluate transfection or treatment conditions, and support fluorescence-based imaging or cell sorting. Applications include screening anti-inflammatory and anticancer agents, studying tumor-associated inflammatory signaling, evaluating transcriptional regulators, and exploring interactions between glioblastoma cells and the immune microenvironment.