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Human SLC6A3 Stable Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01235

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cat. No. CSC-RO01235
Description This cell line is engineered to stably overexpress wild type human solute carrier family 6 member 3 (SLC6A3).
Product Type SLC Transporter Stable Cell Lines
Target Gene SLC6A3
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Size One vial of frozen cells, typically >1x10^6cells/vial
Quality Control 1) Real-Time qPCR
2) Mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene SLC6A3
Background This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]
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Robust Tool

This SLC6A3 (DAT) stable cell line in HEK293 is a high-quality tool for studying dopamine reuptake and transporter pharmacology. The transporter expression is stable and functional, with robust dopamine uptake activity that can be blocked by known inhibitors. It has been a reliable workhorse for our neuropharmacology assays.

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