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Human SLC6A3 Stable Cell Line - HEK293

Human SLC6A3 Stable Cell Line - HEK293

Cat.No. :  CSC-RO01235 Host Cell:  HEK293

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Cat. No. CSC-RO01235
Description This cell line is engineered to stably overexpress wild type human solute carrier family 6 member 3 (SLC6A3).
Gene SLC6A3
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Product Type SLC Transporter Stable Cell Lines
Quality Control 1) Real-Time qPCR
2) Mycoplasma detection
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Size Form One vial of frozen cells, typically >1x10^6cells/vial
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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